Dr Paul Monis

Adjunct Associate Professor UniSA STEM

email Paul.Monis@unisa.edu.au

Research Degree Supervisor

About me

Paul was awarded his PhD in 1997 for his work investigating the molecular systematics of Giardia, which was conducted at the University of Adelaide. In 1998 Paul joined the South Australian Water Corporation as a Research Microbiologist based at the Australian Water Quality Centre. In this position he was responsible for the development of rapid methods for the isolation and identification of Cryptosporidium and Giardia from water. Paul was appointed as the Research Leader of Microbiology in August 2002 and manages the Microbiology Research and Development Group. Paul's experience includes project management, molecular diagnostics, gene technology and evolutionary biology. Current research projects include the investigation of the...

UniSA STEM

Harnessing connections across science, technology, engineering and mathematics, UniSA STEM creates innovative solutions and groundbreaking developments across teaching, learning and research.

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Orcid
Scopus

Research

Research focus

Microbiology
Biological Sciences
Veterinary Sciences
Multidisciplinary
Parasitology

Highlights

Application of rapid detection technologies (real-time PCR, biosensors) for the detection of microbial contaminants in water

Characterisation of the physical processes involved in the inactivation of pathogens in the environment

Identification of novel indicator organisms for pathogens in waste water

Laboratory and field-based evaluation of disinfection technologies for the inactivation of microorganisms of concern to the water industry

Research

Outputs

Research outputs for the last seven years are shown below. To see earlier years visit ORCID or Scopus

Open access indicates that an output is open access.

Chapters

Year	Output
2014	Monis, P, King, B & Keegan, A 2014, 'Removal and inactivation of cryptosporidium from water', in SM Caccio & G Widmer (eds), Cryptosporidium: parasite and disease, Springer Verlag Wien, Austria, ch. 13, pp. 515-552. 8
2012	Thompson, R & Monis, P 2012, 'Giardia - from genome to proteome', in D Rollinson & SI Hay (eds), Advances in Parasitology, Academic Press, US, vol. 78, ch. 2, pp. 57-95. 86 79 3

Journal Articles

Year	Output
2022	Huang, J, Chow, CW, Shi, Z, Fabris, R, Mussared, A, Hallas, G, Monis, P, Jin, B & Saint, CP 2022, 'Stormwater monitoring using on-line UV-Vis spectroscopy', Environmental Science and Pollution Research, vol. 29, pp. 19530-19539. Open access
2021	Donner, E, Zamyadi, A, Jex, A, Short, M, Drigo, B, McCarthy, D, Crosbie, N, Ahmed, W, Mueller, J, Thomas, K, Monis, P, Keegan, A, Ginige, M, Hugenholtz, P, Tyson, G, Hill, K & Blackall, L 2021, 'Wastewater monitoring for SARS-CoV-2', Microbiology Australia, vol. 42, no. 1, pp. 18-22. Open access 2 3 8
2020	Gaget, V, Hobson, P, Keulen, A, Newton, K, Monis, P, Humpage, AR, Weyrich, LS & Brookes, JD 2020, 'Toolbox for the sampling and monitoring of benthic cyanobacteria', Water Research, vol. 169, article no. 115222, pp. 1-13. 10 5
2020	Gunasekera, S, Zahedi, A, O'dea, M, King, B, Monis, P, Thierry, B, Carr, JM & Ryan, U 2020, 'Organoids and bioengineered intestinal models: potential solutions to the Cryptosporidium culturing dilemma', Microorganisms, vol. 8, no. 5, article no. 715, pp. 1-16. Open access 15 13 4
2018	Diéguez, L, Winter, M, Molan, S, Monis, P, King, B & Thierry, B 2018, 'Disposable microfluidic micromixers for effective capture of Cryptosporidium parvum oocysts from water samples', Journal of Biological Engineering, vol. 12, no. 1, article no. 4, pp. 1-8. Open access 4 2
2018	Fallowfield, HJ, Young, P, Taylor, MJ, Buchanan, N, Cromar, N, Keegan, A & Monis, P 2018, 'Independent validation and regulatory agency approval for high rate algal ponds to treat wastewater from rural communities', Environmental Science: Water Research and Technology, vol. 4, no. 2, pp. 195-205. Open access 12 11
2018	Kulkarni, V, Awad, J, Medlock, A, Monis, P, Lau, M, Drigo, B & van Leeuwen, J 2018, 'Field based pilot-scale drinking water distribution system: Simulation of long hydraulic retention times and microbiological mediated monochloramine decay', MethodsX, vol. 5, pp. 684-696. Open access 2 2
2018	Zahedi, A, Gofton, AW, Greay, T, Monis, P, Oskam, C, Ball, A, Bath, A, Watkinson, A, Robertson, I & Ryan, U 2018, 'Profiling the diversity of cryptosporidium species and genotypes in wastewater treatment plants in Australia using next generation sequencing', Science of the Total Environment, vol. 644, pp. 635-648. 33 30 3
2018	Zahedi, A, Monis, P, Gofton, AW, Oskam, CL, Ball, A, Bath, A, Bartkow, M, Robertson, I & Ryan, U 2018, 'Cryptosporidium species and subtypes in animals inhabiting drinking water catchments in three states across Australia', Water Research, vol. 134, pp. 327-340. Open access 43 41 12
2017	Carvajal, G, Branch, A, Sisson, SA, Roser, DJ, van den Akker, B, Monis, P, Reeve, P, Keegan, A, Regel, R & Khan, SJ 2017, 'Virus removal by ultrafiltration: Understanding long-term performance change by application of Bayesian analysis', Water Research, vol. 122, pp. 269-279. 13 12 4
2017	Gaget, V, Humpage, AR, Huang, Q, Monis, P & Brookes, JD 2017, 'Benthic cyanobacteria: a source of cylindrospermopsin and microcystin in Australian drinking water reservoirs', Water Research, vol.124, pp. 454-464. 67 62 7
2017	Gaget, V, Keulen, A, Lau, M, Monis, P & Brookes, JD 2017, 'DNA extraction from benthic cyanobacteria: comparative assessment and optimization', Journal of Applied Microbiology, vol. 122, no. 1, pp. 294-304. Open access 11 8 6
2017	King, B, Fanok, S, Phillips, R, Lau, M, van den Akker, B & Monis, P 2017, 'Cryptosporidium attenuation across the wastewater treatment train: recycled water fit for purpose', Applied and Environmental Microbiology, vol. 83, no. 5, article no. e03068-16, pp. 1-18. Open access 24 19
2017	Lee, E, Khurana, MS, Whiteley, AS, Monis, PT, Bath, A, Gordon, C, Ryan, UM & Paparini, A 2017, 'Novel primer sets for next generation sequencing-based analyses of water quality', PLoS One, vol. 12, no. 1, article no. e0170008, pp. 1-17. Open access 15 14 8
2017	Monis, P, Lau, M, Harris, M, Cook, D & Drikas, M 2017, 'Risk-based management of drinking water safety in Australia: Implementation of health based targets to determine water treatment requirements and identification of pathogen surrogates for validation of conventional filtration', Food and Waterborne Parasitology, vol. 8-9, pp. 64-74. Open access 10 1
2016	Chahal, C, van den Akker, B, Young, F, Franco, C, Blackbeard, J & Monis, P 2016, 'Pathogen and particle associations in wastewater: significance and implications for treatment and disinfection processes', Advances in Applied Microbiology, vol. 97, pp. 63-119. Open access 66 60
2016	Reeve, P, Regel, R, Dreyfus, J, Monis, P, Lau, M, King, B & Van Den Akker, B 2016, 'Virus removal of new and aged UF membranes at full-scale in a wastewater reclamation plant', Environmental Science: Water Research and Technology, vol. 2, no. 6, pp. 1014-1021. 13 12 1
2016	Ryan, U, Paparini, A, Monis, P & Hijjawi, N 2016, 'It's official - Cryptosporidium is a gregarine: what are the implications for the water industry?', Water Research, vol. 105, pp. 305-313. Open access 63 56 2
2016	Sawade, E, Monis, P, Cook, D & Drikas, M 2016, 'Is nitrification the only cause of microbiologically induced chloramine decay?', Water Research, vol. 88, pp. 904-911. 15 15
2016	Watson, SB, Monis, P, Baker, P & Giglio, S 2016, 'Biochemistry and genetics of taste- and odor-producing cyanobacteria', Harmful Algae, vol. 54, pp. 112-127. 119 105 1
2016	Zahedi, A, Monis, P, Aucote, S, King, B, Paparini, A, Jian, F, Yang, R, Oskam, C, Ball, A, Robertson, I & Ryan, U 2016, 'Zoonotic Cryptosporidium species in animals inhabiting Sydney water catchments', PLoS One, vol. 11, no. 12, pp. 1-18. Open access 42 37 5
2015	Hallas, G & Monis, P 2015, 'Evaluation of heterotrophic plate and chromogenic agar colony counting in water quality laboratories', MethodsX, vol. 2, pp. 415-422. Open access 2 1 9
2015	King, B, Fanok, S, Phillips, R, Swaffer, BA & Monis, P 2015, 'Integrated Cryptosporidium assay to determine oocyst density, infectivity, and genotype for risk assessment of source and reuse water', Applied and Environmental Microbiology, vol. 81, no. 10, pp. 3471-3481. Open access 14 14
2015	Li, N, Neumann, NF, Ruecker, N, Alderisio, KA, Sturbaum, GD, Villegas, EN, Chalmers, R, Monis, P, Feng, Y & Xiao, L 2015, 'Development and evaluation of three real-time PCR assays for genotyping and source tracking Cryptosporidium spp. in water', Applied and Environmental Microbiology, vol. 81, no. 17, pp. 5845-5854. Open access 21 21 1
2015	Shaw, JL, Monis, P, Weyrich, LS, Sawade, E, Drikas, M & Cooper, AJ 2015, 'Using amplicon sequencing to characterize and monitor bacterial diversity in drinking water distribution systems', Applied and Environmental Microbiology, vol. 81, no. 18, pp. 6463-6473. Open access 42 39 2
2015	Wielinga, C, Thompson, RA, Monis, P & Ryan, U 2015, 'Identification of polymorphic genes for use in assemblage B genotyping assays through comparative genomics of multiple assemblage B Giardia duodenalis isolates', Molecular and Biochemical Parasitology, vol. 201, no. 1, pp. 1-4. Open access 26 22 1
2015	Yang, R, Ying, JLJ, Monis, P & Ryan, U 2015, 'Molecular characterisation of Cryptosporidium and Giardia in cats (Felis catus) in Western Australia', Experimental Parasitology, vol. 155, pp. 13-18. Open access 33 32 2
2014	King, B & Monis, P 2014, 'Sanitation: thwart fatal infant gut parasite', Medical Journal of Australia, vol. 507, no. 7493, pp. 431-431. Open access 1
2014	Koh, W, Thompson, A, Edwards, H, Monis, P & Clode, PL 2014, 'Extracellular excystation and development of Cryptosporidium: tracing the fate of oocysts within Pseudomonas aquatic biofilm systems', BMC Microbiology, vol. 14, no. 1, article no. 281, pp. 1-12. Open access 29 26 2
2014	Lee, E, Ryan, UM, Monis, P, McGregor, GB, Bath, A, Gordon, C & Paparini, A 2014, 'Polyphasic identification of cyanobacterial isolates from Australia', Water Research, vol. 59, pp. 248-261. Open access 23 21
2014	Shaw, JL, Monis, P, Fabris, R, Ho, L, Braun, K, Drikas, M & Cooper, A 2014, 'Assessing the impact of water treatment on bacterial biofilms in drinking water distribution systems using high-throughput DNA sequencing', Chemosphere, vol. 117, no. 1, pp. 185-192. 38 32 1
2014	Swaffer, BA, Vial, HM, King, BJ, Daly, R, Frizenschaf, J & Monis, PT 2014, 'Investigating source water Cryptosporidium concentration, species and infectivity rates during rainfall-runoff in a multi-use catchment', Water Research, vol. 67, pp. 310-320. 29 30
2014	Tsao, H-W, Michinaka, A, Yen, H-K, Giglio, S, Hobson, P, Monis, P & Lin, T-F 2014, 'Monitoring of geosmin producing Anabaena circinalis using quantitative PCR', Water Research, vol. 49, pp. 416-425. 40 37 1
2014	Weber, M, Sari, IP, Hoefel, D, Monis, P & King, B 2014, 'PCR slippage across the ML-2 microsatellite of the cryptosporidium MIC1 locus enables development of a PCR assay capable of distinguishing the zoonotic cryptosporidium parvum from other human infectious cryptosporidium species', Zoonoses and Public Health, vol. 61, no. 5, pp. 324-337. 5 4 1
2014	Yang, R, Paparini, A, Monis, P & Ryan, U 2014, 'Comparison of next-generation droplet digital PCR (ddPCR) with quantitative PCR (qPCR) for enumeration of Cryptosporidium oocysts in faecal samples', International Journal for Parasitology, vol. 44, no. 14, pp. 1105-1113. Open access 124 113 3
2013	Keegan, A, Robinson, B, Monis, P, Biebrick, M & Liston, C 2013, 'Validation of activated sludge plant performance for virus and protozoan reduction', Journal of Water Reuse and Desalination, vol.3, no. 2, pp. 140-147. Open access 4 2
2013	Koh, W, Clode, PL, Monis, P & Thompson, RA 2013, 'Multiplication of the waterborne pathogen Cryptosporidium parvum in an aquatic biofilm system', Parasites and Vectors, vol. 6, no. 1, article no. 270, pp. 1-12. Open access 38 34
2012	Ho, L, Braun, K, Fabris, R, Hoefel, D, Morran, J, Monis, P & Drikas, M 2012, 'Comparison of drinking water treatment process streams for optimal bacteriological water quality', Water Research, vol. 46, no. 12, pp. 3934-3942. 42 40
2012	Ho, L, Tang, T, Monis, PT & Hoefel, D 2012, 'Biodegradation of multiple cyanobacterial metabolites in drinking water supplies', Chemosphere, vol. 87, no. 10, pp. 1149-1154. 60 49
2012	King, B, Keegan, A, Phillips, R, Fanok, S & Monis, P 2012, 'Dissection of the hierarchy and synergism of the bile derived signal on Cryptosporidium parvum excystation and infectivity', Parasitology (Cambridge), vol. 139, no. 12, pp. 1533-1546. 16 12
2011	Bernard, C, Froscio, S, Campbell, R, Monis, P, Humpage, AR & Fabbro, L 2011, 'Novel toxic effects associated with a tropical Limnothrix/Geitlerinema-like cyanobacterium', Environmental Toxicology, vol. 26, no. 3, pp. 260-270. 26 24
2011	Cai, G, Jin, B, Monis, P & Saint, C 2011, 'Metabolic flux network and analysis of fermentative hydrogen production', Biotechnology Advances, vol. 29, no. 4, pp. 375-387. 97 87
2011	Cai, G, Jin, B, Saint, CP & Monis, P 2011, 'Genetic manipulation of butyrate formation pathways in Clostridium butyricum', Journal of Biotechnology, vol. 155, no. 3, pp. 269-274. 49 49
2011	Giglio, S, Chou, W, Ikeda, H, Cane, D & Monis, P 2011, 'Biosynthesis of 2-methylisoborneol in cyanobacteria', Environmental Science and Technology, vol. 45, no. 3, pp. 992-998. Open access 95 82
2011	Giglio, S, Saint, CP & Monis, PT 2011, 'Expression of the geosmin synthase gene in the cyanobacterium anabaena circinalis AWQC318', Journal of Phycology: An International Journal of Algal Research, vol. 47, no. 6, pp. 1338-1343. 21 17
2011	King, B, Keegan, A, Robinson, B & Monis, P 2011, 'Cryptosporidium cell culture infectivity assay design', Parasitology (Cambridge), vol. 138, nol. 6, pp. 671-681. 20 18
2011	Wielinga, C, Ryan, U, Thompson, RA & Monis, P 2011, 'Multi-locus analysis of Giardia duodenalis intra-Assemblage B substitution patterns in cloned culture isolates suggests sub-Assemblage B analyses will require multi-locus genotyping with conserved and variable genes', International Journal for Parasitology, vol. 41, no. 5, pp. 495-503. Open access 41 40
2010	Hijjawi, N, Estcourt, A, Yang, R, Monis, P & Ryan, U 2010, 'Complete development and multiplication of Cryptosporidium hominis in cell-free culture', Veterinary Parasitology, vol. 169, no. 1-2, pp. 29-36. Open access 31 27
2010	King, BJ, Hoefel, D, Wong, PE & Monis, PT 2010, 'Solar radiation induces non-nuclear perturbations and a false start to regulated exocytosis in Cryptosporidium parvum', PLoS One, vol. 5, no. 7, article no e11773, pp. 1-13. Open access 9 8
2009	Hoefel, D, Ho, L, Monis, P, Newcombe, G & Saint, CP 2009, 'Biodegradation of geosmin by a novel Gram-negative bacterium; isolation, phylogenetic characterisation and degradation rate determination', Water Research, vol. 43, no. 11, pp. 2927-2935. 38 34
2009	King, B, Hoefel, D, Lim, S, Robinson, B & Monis, P 2009, 'Flow cytometric assessment of distinct physiological stages within Cryptosporidium parvum sporozoites post-excystation', Parasitology (Cambridge), vol. 136, no. 9, pp. 953-966. 9 9
2009	Monis, PT, Caccio, SM & Thompson, RA 2009, 'Variation in Giardia: towards a taxonomic revision of the genus', Trends in Parasitology, vol. 25, no. 2, pp. 93-100. 221 195
2009	Wang, X, Monis, P, Saint, CP & Jin, B 2009, 'Biochemical kinetics of fermentative hydrogen production by Clostridium butyricum W5', International Journal of Hydrogen Energy, vol. 34, no. 2, pp. 791-798. 31 26
2008	Boxell, A, Hijjawi, N, Monis, P & Ryan, U 2008, 'Comparison of various staining methods for the detection of Cryptosporidium in cell-free culture', Experimental Parasitology, vol. 120, no. 1, pp. 67-72. 23 20
2008	Giglio, S, Jiang, J, Saint, CP, Cane, DE & Monis, P 2008, 'Isolation and characterization of the gene associated with geosmin production in cyanobacteria', Environmental Science and Technology, vol. 42, no. 21, pp. 8027-8032. Open access 97 87
2008	Hallas, G, Giglio, S, Capurso, V, Monis, P & Grooby, W 2008, 'Evaluation of chromogenic technologies for use in Australian potable water', Journal of Applied Microbiology, vol. 105, no. 4, pp. 1138-1149. Open access 10 11
2008	Jex, A, Smith, H, Monis, P, Campbell, B & Gasser, R 2008, 'Cryptosporidium - biotechnological advances in the detection, diagnosis and analysis of genetic variation', Biotechnology Advances, vol. 26, no. 4, pp. 304-317. 108 89
2008	King, B, Hoefel, D, Daminato, D, Fanok, S & Monis, P 2008, 'Solar UV reduces Cryptosporidium parvum oocyst infectivity in environmental waters', Journal of Applied Microbiology, vol. 104, no. 5, pp. 1311-1323. Open access 71 62

Conferences

Year	Output
2008	Stevens, M, Jex, A, Ferguson, C, Deere, D, Monis, P & Gasser, R 2008, 'Cryptosporidium research and genotyping helps avoid boil water notice in Melbourne', in (eds), Water Quality Technology Conference and Exposition 2008, American Water Works Association, pp. 682-690.

External engagement & recognition

Collaborations

External engagement & recognition

Collaborations

Organisation	Country
Allwater	AUSTRALIA
Australian Water Quality Centre	AUSTRALIA
Brown University	UNITED STATES
Canada Centre for Inland Waters	CANADA
Central Queensland University	AUSTRALIA
Centres for Disease Control and Prevention	UNITED STATES
CH Diagnostic & Consulting Service	UNITED STATES
Cooperative Research Centre for Water Quality and Treatment	AUSTRALIA
Department of Science, Information Technology, Innovation and the Arts	AUSTRALIA
East China University of Science and Technology	CHINA
Ecowise Environmental	AUSTRALIA
Flinders University	AUSTRALIA
Hashemite	JORDAN
Hashemite University	JORDAN
Healthscope Pathology	AUSTRALIA
Henan Agricultural University	CHINA
Huazhong Agricultural University	CHINA
Istituto Superiore di Sanita	ITALY
Kitasato University	JAPAN
Land and Water - CSIRO	AUSTRALIA
Meiho University	TAIWAN
Melbourne Water	AUSTRALIA
Melbourne Water Corporation	AUSTRALIA
Monash University	AUSTRALIA
Murdoch Univeristy	AUSTRALIA
Murdoch University	AUSTRALIA
National Cheng Kung University	TAIWAN
New York City Department of Environmental Protection	UNITED STATES
Queensland University of Technology	AUSTRALIA
SA Water Corporation	AUSTRALIA
Seqwater	AUSTRALIA
Singleton Hospital	UNITED KINGDOM
TRILITY	AUSTRALIA
University if Adelaide	AUSTRALIA
University of Adelaide	AUSTRALIA
University of Alberta	CANADA
University of Calgary	CANADA
University of Melbourne	AUSTRALIA
University of New South Wales	AUSTRALIA
University of Queensland	AUSTRALIA
University of South Australia	AUSTRALIA
University of Western Australia	AUSTRALIA
US Environmental Protection Agency	UNITED STATES
Water Corporation	AUSTRALIA
Water Futures	AUSTRALIA
Water NSW	AUSTRALIA
Water Quality Services	CANADA
Water Research Australia	AUSTRALIA

Teaching & student supervision

Highlights

Paul currently has no direct teaching contact but is happy to supervise students who wish to do work placement projects

Teaching & student supervision

Research degree supervision

Supervisions from 2010 shown

Thesis title	Student status
Smart monitoring for Environmental Microbial Risk Assessment	Current
Evaluating wastewater as an indicator of sporadic protozoan parasitic diseases in humans in South Australia	Completed
Identification, expression, and characterisation of geosmin synthases in cyanobacteria	Completed
Molecular and evolutionary investigations into the cyanotoxin cylindrospermopsin	Completed

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Dr Paul Monis

About me

About me

UniSA STEM

About me

Professional Associations

Research

Research focus

Highlights

Projects

Outputs

Chapters

Journal Articles

Conferences

Current research & highlights

External engagement & recognition

Collaborations

Collaborations

External engagement & recognition

Other

Teaching & student supervision

Highlights

Courses and programs

Research degree supervision

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